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Updated: May 29, 2025
An Orthotopic Murine Model of Human Prostate Cancer Metastasis
Published on: September 18, 2013
Dietary phytochemical indole-3-carbinol regulates metabolic reprogramming in mouse prostate tissue
Rebecca Mary Peter1,2, Md Shahid Sarwar1, Lujing Wang1,2
1Department of Pharmaceutics, Ernest Mario School of Pharmacy, The State University of New Jersey, RutgersPiscataway, NJ, 08854, USA.
Purpose:
Indole-3-carbinol (I3C) is shown to possess multiple pharmacological activities such as anti-inflammatory, antimicrobial, antioxidant, antiviral, and anti-cancer activities. It is widely accepted as modulator of multiple signaling pathways particularly those related to cell cycle, cell growth and division, angiogenesis, apoptosis and immunity. We explored the metabolic reprogramming based on treatment with I3C in mice prostate tissue.
Methods:
In this study we utilized Pten knockout (KO)-induced prostate tumorigenesis mouse model to examine mechanism of action of I3C via metabolic rewiring. Phosphatase and tensin homolog deleted on chromosome 10 (Pten), a tumor suppressor gene is frequently found to be mutated or deleted in prostate cancer. Untargeted metabolomics was performed using liquid-chromatography mass-spectrometry (LC-MS) based platform to investigate Pten-dependent and Pten-independent metabolic targets of I3C.
Results:
The most impacted pathways by I3C included pyrimidine metabolism, arginine and proline metabolism, porphyrin metabolism, citrate cycle and lipoic acid metabolism.
Conclusion:
These pathways taken together help in understanding the overall health beneficial effects of I3C.
