Related Experiment Video
Updated: May 29, 2025

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method
Published on: June 14, 2020
Biomimetic separations in chemistry and life sciences
Fotios Tsopelas1, Chrysanthos Stergiopoulos2, Panagiotis Danias2
1Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Iroon Polytechniou 9, 15780, Zografou Athens, Greece. ftsop@central.ntua.gr.
Abstract:
Since Otto Schmitt introduced the term "biomimetics" in 1957, the imitation of biological systems to develop separation methods and simulate biological processes has seen continuous growth, particularly over the past five decades. The biomimetic approach relies on the use of specific ligands-biospecific, biomimetic, or synthetic-which target biomolecules, such as proteins, antibodies, nucleic acids, enzymes, drugs, pesticides, and other bioactive analytes. This review highlights advances in biomimetic separations, focusing on biomimetic liquid chromatography (including immobilized artificial membrane chromatography, cell membrane chromatography, biomimetic affinity chromatography, weak affinity chromatography, micellar liquid chromatography, immobilized liposome chromatography, and liposome electrokinetic capillary chromatography) for the complex separation and purification of biomolecules and other important chemical compounds. It also explores their application in studying drug-receptor interactions, screening chemical permeability, absorption, distribution, toxicity, as well as predicting environmental risks. Additionally, this review discusses the application of biomimetic magnetic nanoparticles, which leverage biological membranes and proteins for drug discovery, protein purification, and diagnostics.
More Related Videos
13:46Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Published on: September 29, 2011
07:28Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
Published on: November 4, 2021
Related Concept Videos
Affinity Chromatography
Overview Of Cell Separation And Isolation
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...