Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Identification of a family of species-selective complex I inhibitors as potential anthelmintics.

Nature communications·2024

Same author

Encapsulation of <i>Caenorhabditis elegans</i> in Water-in-Water Microdroplets to Study the Worm Viability: Alternative Avenue to Manipulate Microdroplet Environment.

ACS applied materials & interfaces·2023

Same author

An intestinally secreted host factor promotes microsporidia invasion of <i>C. elegans</i>.

eLife·2022

Same author

Identification of enzymes that have helminth-specific active sites and are required for Rhodoquinone-dependent metabolism as targets for new anthelmintics.

PLoS neglected tropical diseases·2021

Same author

Alternative splicing of <i>coq-2</i> controls the levels of rhodoquinone in animals.

eLife·2020

Same author

EPIC: software toolkit for elution profile-based inference of protein complexes.

Nature methods·2019

Search research articles

Related Experiment Video

Updated: May 29, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing.

June H Tan¹, Andrew G Fraser²

¹The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.

Nature Biotechnology

|February 5, 2025

Summary

This summary is machine-generated.

We developed small-molecule sequencing (smol-seq) to quantify metabolites using structure-switching aptamers and DNA sequencing. This method precisely measures metabolite levels and enables parallel detection of multiple targets for advanced metabolomics.

More Related Videos

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Related Experiment Videos

Last Updated: May 29, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

Metabolomics is crucial for understanding biological processes.
Current metabolite quantification methods face challenges in specificity and multiplexing.
There is a need for innovative techniques to analyze complex metabolic profiles.

Purpose of the Study:

To introduce a novel method, small-molecule sequencing (smol-seq), for metabolite quantification.
To demonstrate the utility of structure-switching aptamers (SSAs) in conjunction with DNA sequencing for metabolomics.
To enable high-throughput and specific measurement of metabolites.

Main Methods:

Developed structure-switching aptamers (SSAs) designed to bind specific target metabolites.
Utilized a mechanism where SSA binding releases a unique DNA barcode.
Employed DNA sequencing to read out the released barcodes, correlating them to metabolite concentrations.

Main Results:

Demonstrated high specificity of SSAs for individual metabolite detection.
Showcased the ability to multiplex SSAs for simultaneous detection of multiple metabolites.
Successfully quantified metabolite levels by sequencing the released DNA barcodes.

Conclusions:

smol-seq offers a powerful new approach for metabolite quantification in complex biological samples.
The combination of SSAs and DNA sequencing enhances specificity and multiplexing capabilities in metabolomics.
This method advances the field of metabolomics by providing a sensitive and scalable tool for metabolite analysis.