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Integrated metabolome and transcriptome analysis reveals potential mechanism during the bud dormancy transition of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao

  • 0Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China.
Frontiers in Plant Science +

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February 5, 2025

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February 5, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Understanding bud dormancy in Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao (AMM) is key for cultivation. This study reveals carbohydrate metabolism and IAA signaling regulate dormancy transitions, offering strategies to shorten breeding time.

Area Of Science

  • Plant Science
  • Molecular Biology
  • Agricultural Science

Background

  • Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao (AMM) is a vital medicinal and food plant cultivated via rhizome transplantation.
  • Understanding AMM bud dormancy is crucial for optimizing harvest and propagation, yet it remains understudied in perennial herbs.

Purpose Of The Study

  • Investigate the molecular mechanisms governing the transition from endodormancy to ecodormancy in AMM underground buds.
  • Identify key metabolic pathways and genes involved in dormancy regulation.

Main Methods

  • Simultaneous transcriptome and non-targeted metabolome analyses of dormant AMM buds at different stages.
  • Differential gene expression (DEG) and differentially accumulated metabolite (DAM) analysis.
  • Functional enrichment, co-expression network analysis, and transcription factor identification.

Main Results

  • 1,069 DAMs and 16,832 DEGs were identified, primarily involved in amino acid and carbohydrate metabolism.
  • Carbohydrate metabolism (starch and sucrose) and hormone signaling pathways, particularly indole-3-acetic acid (IAA), were critical for dormancy transitions.
  • Candidate genes (e.g., BGL, GN, glgC, glgB) and transcription factors (MYB, ERF, bHLH) regulating these processes were identified.

Conclusions

  • The study elucidates a novel regulatory mechanism for endo- to ecodormancy transition in AMM buds, involving starch/sucrose metabolism and IAA signaling.
  • Findings provide insights for advanced dormancy breaking strategies and accelerated breeding in AMM.

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