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Updated: May 29, 2025
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Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery
Published on: September 5, 2018
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Acetic acid enabled nuclear contrast enhancement in epi-mode quantitative phase imaging
Zhe Guang1, Amunet Jacobs1,2, Paloma Casteleiro Costa3
1Emory University, Georgia Institute of Technology, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia, United States.
Journal of Biomedical Optics
|February 5, 2025
Summary
Acetic acid (AA) enhances nuclear contrast in quantitative phase imaging (QPI) by altering refractive index. A novel microscopy technique and AI successfully created virtually stained images, advancing label-free histology.
Area of Science:
- Biomedical Optics
- Quantitative Phase Imaging
- Histopathology
Background:
- Acetic acid (AA) acetowhitening enhances nuclear light scattering for tissue inspection.
- Changes in refractive index during acetowhitening also impact nuclear contrast in quantitative phase imaging (QPI).
Purpose of the Study:
- To explore refractive index changes during acetowhitening using quantitative oblique back-illumination microscopy (qOBM).
- To demonstrate machine learning for converting qOBM images into virtually AA-stained images.
Main Methods:
- Implemented qOBM for epi-mode 3D QPI of thick tissue samples, focusing on AA-induced nuclear contrast.
- Applied a Cycle-GAN algorithm to convert qOBM images into virtually AA-stained images.
Main Results:
- Acetowhitening significantly enhanced nuclear contrast in qOBM images of thick mouse brain tissue.
- The Cycle-GAN algorithm successfully simulated AA staining, enhancing nuclear features without physical stains.
Conclusions:
- Acetowhitening-induced refractive index changes enhance nuclear contrast in QPI.
- qOBM combined with virtual staining offers a promising label-free and slide-free approach for ex vivo and in vivo histology.
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