Related Experiment Video
Updated: May 29, 2025

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
Multiple Mutations-A Genetic Marker for Extracapsular Spread in Human Papillomavirus/p16-Positive Oropharyngeal
Raphaela Graessle1,2,3, Iris Piwonski4, Cora Husemann4
1Department of Otorhinolaryngology - Head and Neck Surgery University Hospital Ruppin-Brandenburg, Brandenburg Medical School Theodor Fontane (MHB) Neuruppin Germany.
Background:
In the 8th edition of the TNM classification, extracapsular spread (ECS) became a factor in classifying the UICC stage of oropharyngeal carcinomas (OPSCC). We aimed to find genetic markers for ECS and to identify differences between HPV/p16-positive and HPV/p16-negative cases.
Methods:
We performed targeted next-generation sequencing on 99 samples of operable OPSCC and a retrospective analysis of clinical data.
Results:
We included 55 HPV/p16-positive and 44 HPV/p16-negative patients. We found a significant difference between both groups, particularly in TP53 mutation (p < 0.001). Among other things, a small primary tumor (p < 0.001), no ECS (p = 0.026) were identified as predictors for survival. Multiple mutations were associated with an increased incidence of ECS, especially in HPV+/p16+ cases (p = 0.017). A mutation in PIK3CA occurred more frequently in nonsmokers, especially in HPV-/p16- patients (p = 0.027). A PTEN mutation-which only occurred in HPV+/p16+ tissues-reduced disease-free survival (DFS, p = 0.026).
Conclusion:
The presence of multiple mutations in HPV+/p16+ OPSCC was associated with a higher risk of ECS.
Level Of Evidence:
3.
More Related Videos
06:57Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Published on: June 14, 2019
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017
Related Concept Videos
Cancers Originate from Somatic Mutations in a Single Cell
Non-LTR Retrotransposons
Abnormal Proliferation
Induced Pluripotent Stem Cells
Somatic...