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Recent Developments in SERS Microfluidic Chips: From Fundamentals to Biosensing Applications.

Shuai Lian¹, Xiaoqiong Li¹, Xuefei Lv¹

¹School of Medical Technology, Beijing Institute of Technology, Beijing 100000, China.

ACS Applied Materials & Interfaces

|February 5, 2025

Summary

Surface-enhanced Raman spectroscopy (SERS) microfluidic chips offer sensitive and selective biosensing. This review details their progress, challenges, and future potential in detecting various biological targets.

Keywords:

Biomarkers Biosensors Microfluidic Chips Nanotechnology Nucleic Acids Optical Detectors Proteins Surface-Enhanced Raman Spectroscopy

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Area of Science:

Analytical Chemistry
Biotechnology
Microfluidics

Background:

Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity and selectivity for biosensing.
Challenges in SERS include signal stability and reproducibility due to environmental factors.
Microfluidic chips provide efficient sample handling and on-chip detection capabilities.

Purpose of the Study:

To review the latest research progress of SERS microfluidic chips in biosensing.
To discuss the integration of SERS with microfluidics for enhanced biosensing performance.
To explore SERS microfluidic applications for various biological targets.

Main Methods:

Introduction to SERS enhancement mechanisms and SERS strategies (labeled and label-free).
Categorization of SERS microfluidic chips (colloidal nanoparticle-based and fixed substrate-based).
Review of recent advancements in detecting nucleic acids, proteins, small biomolecules, and live cells.

Main Results:

SERS microfluidic chips combine the advantages of SERS and microfluidics for improved biosensing.
Demonstrated applications in detecting diverse biological targets with high sensitivity and selectivity.
Identified key challenges and proposed solutions for enhancing stability and reproducibility.

Conclusions:

SERS microfluidic chips represent a promising technology for advanced biosensing applications.
Addressing current challenges will further unlock the potential of this combined technology.
Future development outlook focuses on optimizing performance and expanding application scope.