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Cryopreserved sperm does not affect larval ontogeny and quality in Rhamdia quelen
Vanessa Conceição Coimbra1,2,3, Jeane Rodrigues1,2,3, Raquel Santos Dos Santos1,2,4
1Group of Studies On the Reproduction of Amazon Fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil.
Fish Physiology and Biochemistry
|February 5, 2025
Summary
Cryopreservation impacts fish sperm quality, reducing motility and fertilization rates. However, successfully fertilized eggs from cryopreserved sperm develop into normal Rhamdia quelen larvae, ensuring species conservation and aquaculture potential.
Area of Science:
- Aquaculture and Conservation Biology
- Reproductive Biology
- Fish Physiology
Background:
- Cryopreservation of fish sperm is vital for aquaculture and conserving endangered species.
- Cryopreservation can negatively affect sperm quality and offspring viability.
Purpose of the Study:
- To assess the larval development of jundiá (Rhamdia quelen) derived from cryopreserved sperm.
- To compare larval development from cryopreserved sperm versus fresh sperm.
Main Methods:
- Sperm was cryopreserved using a solution of skimmed milk powder, methanol, and fructose.
- Oocytes were fertilized with either fresh or cryopreserved/thawed sperm.
- Larval development was monitored for 25 days post-hatching, with regular sampling.
Main Results:
- Cryopreservation significantly reduced sperm motility, progressivity, and beat cross frequency.
- Fertilization rates were lower with cryopreserved sperm, but hatching rates showed no significant difference.
- Larvae from both fresh and cryopreserved sperm exhibited similar initial morphological development and patterns.
Conclusions:
- The cryopreservation protocol negatively impacts Rhamdia quelen sperm quality.
- Despite reduced sperm quality, cryopreserved sperm can still produce viable offspring with normal larval development.
- This supports the utility of sperm cryopreservation for Rhamdia quelen aquaculture and conservation efforts.
