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Updated: May 29, 2025
Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
Published on: July 14, 2023
Enhanced osteogenic differentiation in hyaluronic acid methacrylate (HAMA) matrix: a comparative study of hPDC and
Ane Albillos Sanchez1, Filipa Castro Teixeira1, Paula Casademunt2
1Complex Tissue Regeneration Department, Maastricht University, MERLN Institute for Technology-Inspired Regenerative Medicine, 6229 ER Maastricht, The Netherlands.
Abstract:
Bone tissue engineering (BTE) seeks to overcome the limitations of traditional bone repair methods, such as autografts and allografts, which are often limited by availability, donor-site morbidity, immune rejection, and infection risks. Recent advancements have highlighted the potential of spheroids or microtissues as building blocks for BTE. This study aimed to investigate the osteogenic differentiation of spheroids formed from human periosteum-derived cells (hPDCs) and bone marrow-derived mesenchymal stromal cells (hBMSCs) in a hyaluronic acid methacrylate (HAMA) matrix, using encapsulation and extrusion bioprinting methods. Results showed significant morphological changes, high viability, and osteogenic differentiation of spheroids from hPDCs or hBMSCs in three-dimensional HAMA environments. Notably, hPDC spheroids demonstrated higher mineralization capabilities and superior hydrogel colonization than hBMSC spheroids. These findings reveal the potential of HAMA bioink containing hPDC spheroids to produce mineralized bone grafts using a bioprinting approach.
