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Enhanced polyhydroxyalkanoate biosynthesis by Cupriavidus sp. CY-1 utilizing CO2 under controlled non-explosive
Young-Cheol Chang1, M Venkateswar Reddy2, Yasuteru Mawatari1
1Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, 050-8585, Japan.
Chemosphere
|February 5, 2025
Summary
This study demonstrates efficient polyhydroxyalkanoate (PHA) production using hydrogen-oxidizing bacteria and carbon dioxide (CO2). Cupriavidus sp. CY-1 achieved high PHA yields, showcasing potential for sustainable bioplastic manufacturing.
Area of Science:
- Biotechnology and microbial engineering
- Polymer science and sustainable materials
Background:
- Polyhydroxyalkanoates (PHAs) are biodegradable polyesters with diverse applications.
- Sustainable production of PHAs using carbon dioxide (CO2) and hydrogen (H2) is an active research area.
- Optimizing fermentation conditions is crucial for efficient PHA biosynthesis.
Purpose of the Study:
- To evaluate the PHA production capacity of Cupriavidus sp. CY-1 under various non-explosive gaseous conditions.
- To investigate the impact of different gas compositions and additives on PHA yield and productivity.
- To explore strategies for enhancing PHA production and copolymer synthesis.
Main Methods:
- Cultivation of Cupriavidus sp. CY-1 using a mixed gas substrate (H2, O2, CO2).
- Optimization of fermentation parameters, including gas concentrations and addition of Tween 80.
- Two-step fermentation for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using valeric acid.
- Analysis of phaC gene expression to correlate with PHA productivity.
Main Results:
- Cupriavidus sp. CY-1 achieved a high PHA production rate of 11.87 g L-1 with a 90.6% yield using a H2/O2/CO2 mixture.
- Optimal non-explosive conditions were identified, with specific H2 and O2 concentrations investigated.
- CO addition negatively impacted growth and PHA production, while Tween 80 enhanced productivity.
- Successful synthesis of PHBV at 1.47 g L-1 was achieved through a two-step process.
- PHA productivity was predictable based on phaC gene expression levels.
- CO2 derived from phenol biodegradation showed significant potential for PHA production.
Conclusions:
- Cupriavidus sp. CY-1 is a highly efficient strain for PHA biosynthesis using CO2 and H2 under safe conditions.
- Optimized fermentation strategies and the use of specific substrates can significantly enhance PHA and PHBV production.
- Gene expression analysis provides valuable insights for predicting PHA yields.
- Utilizing CO2 from biodegradation processes offers a promising route for sustainable bioplastic manufacturing.
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