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Updated: May 29, 2025
Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Functional study of tilapia T cell activation stimulus signal molecule CD2
Yuan Li1, Zhi-Wen Wang2, Bei Wang2
1Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, 524088, Zhanjiang, Guangdong, China; College of Biology and Agriculture, Shaoguan University, 512005, Shaoguan, China.
Abstract:
As a co-stimulatory signaling molecule, CD2 provides a secondary stimulatory signal during T-cell activation. Research on CD2 in lower vertebrates remains limited. In this article, we identify the tilapia CD2 gene (GenBank accession number: OM974656; designated as OnCD2), which is predominantly expressed in various immune tissues of tilapia. Additionally, we determine the interaction between tilapia CD2 and its ligand, CD48. Using the magnetic bead method, we identified the CD2-positive lymphocyte population in tilapia, which primarily includes Th, NCC, and B cells. Under different stimuli, the proportion of CD2-positive cells in the head kidney, peripheral blood, and spleen lymphocytes exhibited varying degrees of increase. Incubation of tilapia lymphocytes with CD2 antibody and CD48 recombinant protein upregulated the expression of genes associated with T cell activation. The results of this study demonstrate that tilapia CD2 not only plays a role in T cell activation through its interaction with the ligand CD48, but also mediates a more complex immune regulation pathway in tilapia. This research serves as a reference for the classification of fish immune cells and enhances our understanding of T cell immunity in fish.
Insights
Researchers identified the tilapia CD2 gene, crucial for T-cell activation. This study reveals CD2
Area of Science:
- Immunology
- Fish Biology
- Molecular Biology
Background:
- The role of CD2, a co-stimulatory molecule in T-cell activation, is understudied in lower vertebrates.
- Limited research exists on CD2 function and interactions in fish immune systems.
Purpose of the Study:
- To identify and characterize the tilapia CD2 gene (OnCD2).
- To investigate the interaction between tilapia CD2 and its ligand, CD48.
- To elucidate the role of OnCD2 in T-cell activation and immune regulation in tilapia.
Main Methods:
- Gene identification and sequencing of tilapia CD2.
- Expression analysis in various tilapia immune tissues.
- Magnetic bead-based identification of CD2-positive lymphocytes.
- Flow cytometry to assess changes in CD2-positive cell populations.
- Gene expression analysis following stimulation with CD2 antibody and CD48 recombinant protein.
Main Results:
- The tilapia CD2 gene (OnCD2) was identified and found to be predominantly expressed in immune tissues.
- CD2-positive lymphocytes in tilapia include T helper (Th) cells, natural cytotoxic (NCC) cells, and B cells.
- Stimulation with CD2 antibody and CD48 recombinant protein upregulated genes associated with T-cell activation.
- The proportion of CD2-positive cells increased in head kidney, peripheral blood, and spleen lymphocytes under various stimuli.
Conclusions:
- Tilapia CD2 plays a significant role in T-cell activation via interaction with CD48.
- OnCD2 is involved in a complex immune regulation pathway in tilapia.
- This study provides a reference for fish immune cell classification and advances understanding of fish T-cell immunity.
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