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Updated: May 29, 2025
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Controlling Multidrug-Resistant Staphylococcus aureus by Combined Use of Antimicrobials and Phage STPX-6 with Broad
Objective:
The emergence of antibiotic-resistant Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), poses a great challenge for animal and public health. This study aimed to isolate a broad-spectrum and high-efficiency MRSA phage and explore the phage-antibiotic synergistic effect on MRSA.
Results:
Phage STPX-6 belongs to Caudovirales, Podoviridae. It has a hexahedral head and a short tail. Its genome length was 17,007 bp, and it did not contain resistance genes and virulence genes. STPX-6 lysed 79.6% (133/167) of 167 S. aureus and 87.96% (95/108) of MRSA from different sources. The titer of phage was 1.18 × 1010 PFU/mL, the optimal multiplicity of infection was 1, the latent period and lysis period were about 10 min and 60 min, respectively, and the burst amount was 68 PFU/cell. At 50°C and 70-90°C, the titer of STPX-6 was maintained at about 1010 PFU/mL and at least 103 PFU/mL, respectively. In the range of pH 4-12, the titer of phage remained above 108 PFU/mL, and it remained above 104 PFU/mL at pH 2, 3, 13, and 14. The combined application of phage STPX-6 and enrofloxacin, doxycycline, ampicillin could reduce the minimum inhibitory concentration (MIC) of the three antibiotics to 1/4 MIC, 1/16 MIC, and 1/2 MIC, respectively.
Conclusion:
This study found that for the host MRSA, lytic phage STPX-6 had the characteristics of a broad lytic spectrum, a short latent period, strong adaptability and strong tolerance to high temperature, a strong acid and strong alkali environment, and might maintain certain activity under extreme environment. More importantly, the combination of phage STPX-6 with enrofloxacin, doxycycline, and ampicillin could reduce the antibiotic concentration used for MRSA. In other words, phages as new antibacterial agents have received increasing attention. The combined application of phages and antibiotics provides a new method for controlling multidrug resistant bacteria and reduce the use of antibiotics.
Insights
A novel lytic bacteriophage, STPX-6, effectively targets methicillin-resistant Staphylococcus aureus (MRSA). Combining STPX-6 with antibiotics significantly reduces their required concentrations, offering a new strategy against multidrug-resistant bacteria.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Antibiotic resistance in *Staphylococcus aureus*, particularly methicillin-resistant *S. aureus* (MRSA), presents a significant global health challenge.
- The need for alternative therapeutic strategies to combat multidrug-resistant bacteria is critical.
Purpose of the Study:
- To isolate and characterize a broad-spectrum, high-efficiency MRSA bacteriophage.
- To investigate the synergistic effects of the isolated phage in combination with conventional antibiotics against MRSA.
Main Methods:
- Isolation and characterization of bacteriophage STPX-6, including genomic analysis.
- Determination of phage lytic activity against various *S. aureus* and MRSA strains.
- Assessment of phage stability under different temperature and pH conditions.
- Evaluation of phage-antibiotic synergy by measuring reductions in minimum inhibitory concentrations (MICs).
Main Results:
- Phage STPX-6, a *Caudovirales* family member, demonstrated broad lytic activity against 79.6% of *S. aureus* and 87.96% of MRSA strains.
- STPX-6 exhibited robust stability across a wide range of temperatures (up to 90°C) and pH values (2-14).
- Combined application of STPX-6 with enrofloxacin, doxycycline, and ampicillin reduced antibiotic MICs by 1/4, 1/16, and 1/2, respectively.
Conclusions:
- Lytic phage STPX-6 is a promising candidate for MRSA treatment due to its broad spectrum, efficiency, and stability.
- Phage-antibiotic synergy offers a viable approach to reduce antibiotic usage and combat multidrug-resistant bacteria.
- This study highlights the potential of bacteriophages as novel antibacterial agents in animal and public health.
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