Granule cell dendritic spine density in the rat hippocampus varies with spine shape and location

The number of granule cell dendritic spines per micrometer of dendritic length in the dorsal and ventral leaves of the dentate gyrus was quantified using light microscopic-Golgi preparations of normal adult rats. Spines were counted in terms of 3 categories of spine form for the 3 afferent termination zones of the molecular layer and corrected for shading errors. Total spine density averaged 1.6 spines/micron of dendritic length in the dorsal leaf and 1.3 spines/micron of dendritic length in the ventral leaf. Statistically significant differences in spine density existed among the 3 shape categories. Variations in spine density occurred by shape category among the afferent termination zones.