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  1. Home
  2. Sparse Testing Designs For Optimizing Resource Allocation In Multi-environment Cassava Breeding Trials.
  1. Home
  2. Sparse Testing Designs For Optimizing Resource Allocation In Multi-environment Cassava Breeding Trials.

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Sparse testing designs for optimizing resource allocation in multi-environment cassava breeding trials.

Nelson Lubanga1, Beatrice E Ifie1, Reyna Persa2

  • 1Insitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.

The Plant Genome
|February 6, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Sparse testing in cassava breeding can reduce phenotyping costs for multi-environment trials (METs). Implementing models with genotype-by-environment interaction (G × E) improves predictive ability, suggesting fewer overlapping genotypes are needed for training.

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Area of Science:

  • Agricultural Science
  • Plant Breeding
  • Genetics

Background:

  • Developing improved crop cultivars necessitates multi-environment trials (METs) to assess genotype performance across diverse conditions.
  • High phenotyping costs in METs restrict the evaluation of numerous genotypes in all target environments.

Purpose of the Study:

  • To investigate the effectiveness of sparse testing strategies in cassava breeding programs for reducing phenotyping expenses in METs.
  • To evaluate prediction models incorporating genomic data and genotype-by-environment interaction (G × E) for optimizing sparse testing designs.

Main Methods:

  • Utilized a population of 435 cassava genotypes evaluated across five Nigerian environments for dry matter and fresh root yield.
  • Developed sparse testing designs based on non-overlapping (NOL) and completely overlapping (OL) genotype allocations.
  • Assessed three prediction models: one phenotype-only and two incorporating genomic data, with and without G × E modeling.
  • Main Results:

    • All models demonstrated higher predictive ability and lower mean square error (MSE) with larger training datasets.
    • Modeling G × E significantly improved predictive ability and reduced MSE for given training set sizes.
    • Increased OL genotypes led to decreased predictive ability and increased MSE, indicating a need for minimal OL genotypes in training sets.

    Conclusions:

    • Sparse testing, particularly when incorporating G × E, offers a viable approach to reduce phenotyping costs in cassava METs.
    • Optimizing the size and distribution of training populations in sparse testing can enhance predictive ability and cost-efficiency.
    • Integrating crop growth models (CGMs) with genomic prediction presents future potential for further improving predictive accuracy.