Eco-friendly utilization and microbiological characteristics of coal gangue substrate via functional microbial
Junhao Shi1, Xiaoyu Zhang1, Mengting Lu1
1State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.
Abstract:
The development of the coal mining industry increased the production of coal gangue that was one of the most common solid wastes in China. In this study, a simple in situ treatment of gangue was developed. Without the addition of exogenous soil, through composting by using Acinetobacter lwoffii XAQ2297 with a small amount of corn stover powder, the gangue was as a substrate for planting alfalfa. Moreover, the proportion of the formulation was optimized. Results showed that compared with the control group, A. lwoffii XAQ2297 could increase the release of humic acid from gangue. The best growth of alfalfa was achieved when the ratio of gangue to corn stover powder was 9:1, which was able to increase the survival rate of alfalfa to more than 50%. The analysis of substrate properties indicated that urease was the main positive influencing factor, sulfate ions were the main negative influencing factors. In the diversity analysis, Bacillus and Herbinix were the main genera that play a positive role, whereas Pseudomonas and Brevundimonas were the genera involving the main negative influence. This study provides a new strategy for gangue matrixification and reduces the cost of gangue utilization.
