Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Packaging00:58

DNA Packaging

Overview
Curing Methods01:26

Curing Methods

Concrete members with a small surface-to-volume ratio are cured by oiling and moistening the forms before casting the concrete member. These forms can be left in place for a prolonged period to prevent moisture loss, and can be wetted if made of a material suitable for wetting. If the forms are removed early, the concrete member is moistened and covered with polythene sheets to maintain moisture. For large horizontal concrete surfaces exposed to dry weather, a temporary covering is suspended...
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
Pasteurization and Food Preservation01:28

Pasteurization and Food Preservation

Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...
Methods of Controlling Food Spoilage01:26

Methods of Controlling Food Spoilage

Food spoilage is caused by microbial growth or by chemical and physical changes, all of which affect the taste, texture, and safety of food.Temperature-Based PreservationRefrigeration at 0–4 °C slows microbial growth and enzyme activity, making it ideal for short-term storage. However, certain spoilage organisms—such as psychrotrophs like Listeria monocytogenes—can still proliferate at these temperatures. Freezing below -18 °C further slows biological processes by forming ice crystals, which...
Production of Biopesticides01:18

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgical and Transcatheter Approach of a Failed Mitral Valve Repair: A Comprehensive Review on Selecting the Most Suitable Approach.

Journal of clinical medicine·2026
Same author

Preliminary Outcome of Transcatheter Aortic Valve Implantation at Centers Without On-Site Cardiac Surgery.

Journal of cardiovascular development and disease·2026
Same author

The expertise effect: how advancing skills in frozen elephant trunk have broadened surgical indication for acute dissection.

The Journal of cardiovascular surgery·2026
Same author

When Mitral Repair Fails: Understanding Recurrence, Risk Factors, and Treatment Choices.

Journal of cardiovascular development and disease·2026
Same author

Correlation between frailty status, surgical access, and outcomes in older adults with valvular heart disease undergoing cardiac surgery.

npj aging·2026
Same author

Microinvasive, off-pump, transventricular neochordae implantation in recurrent mitral valve regurgitation after open heart surgical repair.

The Journal of thoracic and cardiovascular surgery·2026

Related Experiment Video

Updated: Jul 24, 2026

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement
14:14

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

Published on: December 11, 2017

14.0K

Mitral Annular Calcification: The "Pack and Seal" Technique.

Carlotta Brega1, Marta Di Carlo2, Costanza Fiaschini2

  • 1Cardiovascular Department, Maria Cecilia Hospital, GVM Care & Research, Cotignola, Italy.

Heart, Lung & Circulation
|February 6, 2025
PubMed
Summary

The "Pack and Seal" technique offers a safe surgical approach for mitral valve replacement in patients with mitral annular calcification. This method achieved favorable in-hospital outcomes with no operative deaths.

Keywords:
Calcified annulusMACMitral annular calcificationMitral valve replacement

More Related Videos

A Simplified Stepwise Approach to Echo Guidance during Percutaneous Mitral Valve Repair
08:31

A Simplified Stepwise Approach to Echo Guidance during Percutaneous Mitral Valve Repair

Published on: October 16, 2021

3.8K
Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels
08:12

Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels

Published on: May 26, 2023

1.5K

Related Experiment Videos

Last Updated: Jul 24, 2026

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement
14:14

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

Published on: December 11, 2017

14.0K
A Simplified Stepwise Approach to Echo Guidance during Percutaneous Mitral Valve Repair
08:31

A Simplified Stepwise Approach to Echo Guidance during Percutaneous Mitral Valve Repair

Published on: October 16, 2021

3.8K
Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels
08:12

Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels

Published on: May 26, 2023

1.5K

Area of Science:

  • Cardiology
  • Cardiac Surgery

Background:

  • Mitral annular calcification (MAC) presents significant surgical challenges.
  • A novel calcium-respecting approach is needed for safe mitral valve replacement (MVR).

Purpose of the Study:

  • To evaluate the safety and efficacy of the "Pack and Seal" technique for MVR in patients with MAC.
  • To report in-hospital outcomes of this surgical approach.

Main Methods:

  • The "Pack and Seal" technique was employed for MVR in nine patients with MAC.
  • Patient demographics, surgical details, and postoperative outcomes were analyzed.

Main Results:

  • No operative or 30-day mortality was observed.
  • No postoperative leakages or reoperations for bleeding occurred.
  • One stroke and two pacemaker implantations were reported with no major sequelae.

Conclusions:

  • The "Pack and Seal" technique is a safe and reproducible surgical option for MVR in the presence of MAC.
  • Favorable in-hospital results support the use of this technique.