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Evaluation of Coagulation and Fibrinolysis Mechanisms in Deep Vein Thrombosis Using Clot-Fibrinolysis Waveform
Akiharu Yoshioka1, Daiki Shimomura1, Mayuka Morita1,2
1Department of Laboratory Medicine, Tenri Hospital, Tenri, Japan.
Insights
Deep vein thrombosis (DVT) patients exhibit distinct coagulation patterns compared to healthy individuals. Clot-fibrinolysis waveform analysis (CFWA) reveals differences in the coagulation phase, indicating higher coagulability in DVT.
Area of Science:
- Hematology
- Vascular Medicine
- Diagnostic Assays
Background:
- Deep vein thrombosis (DVT) presents significant health risks, yet its coagulation and fibrinolysis mechanisms require further elucidation.
- Clot-fibrinolysis waveform analysis (CFWA) offers a novel global assay for evaluating these complex hemostatic processes.
Purpose of the Study:
- To investigate the hemostatic mechanisms in deep vein thrombosis (DVT) using clot-fibrinolysis waveform analysis (CFWA).
- To compare coagulation and fibrinolysis profiles between DVT patients and non-DVT individuals.
Main Methods:
- Utilized ultrasonography for DVT diagnosis in 39 patients and 56 controls.
- Performed activated partial thromboplastin time-based CFWA, analyzing first-derivative curves.
- Compared curve shapes across coagulation and fibrinolysis phases between DVT and non-DVT groups.
Main Results:
- Significant differences observed in the coagulation phase first-derivative curves between DVT and non-DVT groups.
- DVT group exhibited sharper, narrower coagulation curves with lower height and width parameters.
- No significant differences were found in the fibrinolysis phase between the groups.
Conclusions:
- CFWA demonstrated distinct coagulation phase patterns in DVT patients, suggesting heightened coagulability.
- CFWA may serve as a valuable tool for assessing the coagulability status in individuals with DVT.
Introduction:
Deep vein thrombosis (DVT) is a common condition associated with significant morbidity and mortality. However, the detailed mechanisms of coagulation and fibrinolysis in DVT have not been adequately investigated. Recently, clot-fibrinolysis waveform analysis (CFWA) has been developed to assess coagulation and fibrinolysis reactions as one global assay. This study aimed to investigate the mechanisms of DVT using CFWA.
Methods:
DVT diagnosis and definition were conducted by the ultrasonography finding, and the numbers of patients of confirmed DVT and non-DVT were 39 and 56, respectively. Activated partial thromboplastin time-based CFWA was conducted, and the first-derivative curves were analyzed. The curves were separated into two phases: coagulation and fibrinolysis, and the curve shapes were compared between the two groups.
Results:
The shapes of first-derivative curves in the coagulation phase for the DVT group were significantly different from those of the non-DVT group, and the curves of the DVT group were sharper and narrower than those of the non-DVT group. Both parameters indicating height and width in the DVT group were significantly lower than the non-DVT group. However, no significant differences were observed in the fibrinolysis phase between two groups.
Conclusion:
The DVT group showed different first-derivative curves in the coagulation phase compared to the non-DVT group in the CFWA assay, suggesting that the DVT group had a higher coagulability status. CFWA could be a useful tool for identifying coagulability status in patients with DVT.
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