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The FRET-Based APTA Sensor/Cy3 Complex for Glucose Determination.

Ghasem Rezanejade Bardajee¹, Afsaneh Rahimi Chahrogh², Mojdeh Atashkadi²

¹Department of Polymer and Materials Chemistry, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran.

Luminescence : the Journal of Biological and Chemical Luminescence

|February 7, 2025

Summary

A new Förster Resonance Energy Transfer (FRET) fluorescent probe using aptamers and quantum dots offers sensitive, cost-effective blood glucose monitoring. This biosensor shows high selectivity and accuracy in biological samples.

Keywords:

CdTe QDs Cy3 FRET mechanism aptamer fluorescent assay glucose detection

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Nanotechnology

Background:

Accurate blood glucose monitoring is crucial for diabetes management.
Existing methods may lack sensitivity, cost-effectiveness, or specificity.
Förster Resonance Energy Transfer (FRET) offers a sensitive detection mechanism.

Purpose of the Study:

To develop a sensitive and cost-effective fluorescent probe for blood glucose monitoring.
To utilize a FRET-based system incorporating aptamers and quantum dots.
To evaluate the probe's performance in biological samples.

Main Methods:

Development of a FRET sensor using Cadmium Telluride-Thioglycolic Acid Quantum Dots (CdTe-TGA QDs) and a Cy3-labeled aptamer.
Investigation of fluorescence quenching by glucose molecules.
Characterization using UV-Vis, FT-IR, XPS, FESEM, EDX, TEM, AFM, and DLS.

Main Results:

The probe achieved a low detection limit of 7.72 × 10^-9 M for glucose.
Demonstrated excellent selectivity and specificity for glucose.
Showed high recovery rates (96.00-101.11%) in human serum and urine.

Conclusions:

The developed FRET-based APTA sensor/Cy3 complex is a sensitive and reliable tool for glucose detection.
This probe has potential for clinical diagnostics and biological metabolite screening.
The study highlights the utility of quantum dot-aptamer conjugates in biosensing applications.