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High-Throughput Proteomics Reveals a Novel Small Open Reading Frame-Encoded Peptide That Promotes Hepatocellular
Fei Wang1,2,3, En Hu1,2, Juping Li1,2
1The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China.
Journal of Proteome Research
|February 7, 2025
Summary
Researchers identified novel small open reading frame-encoded peptides (SEPs) in hepatocellular carcinoma (HCC). One peptide, LINC01007-33AA, is upregulated in HCC and promotes cancer cell invasion and migration.
Area of Science:
- Molecular Biology
- Oncology
- Proteomics
Background:
- Long noncoding RNAs (lncRNAs) are implicated in tumor development.
- Small open reading frames (smORFs) within lncRNAs can encode peptides (SEPs).
Purpose of the Study:
- To investigate the expression profile of SEPs in hepatocellular carcinoma (HCC).
- To identify novel functional peptides associated with HCC progression.
Main Methods:
- High-throughput mass spectrometry (MS) was used to analyze SEP expression in 154 HCC patients' tumor and adjacent nontumor tissues.
- Quantitative real-time PCR and Western blotting were employed for confirmation.
Main Results:
- A total of 208 SEPs were identified, with comparable abundance and stability to coding region proteins.
- The peptide LINC01007-33AA was significantly upregulated in HCC tissues (p < 0.05).
- Overexpression of LINC01007-33AA enhanced HCC cell invasion and migration and served as an independent risk factor for prognosis.
Conclusions:
- This study highlights the potential of MS-based methods for discovering functional peptides encoded by noncoding sequences.
- LINC01007-33AA represents a potential biomarker and therapeutic target for HCC.
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