CINNAMON-GUI: Revolutionizing Pap Smear Analysis with CNN-Based Digital Pathology Image Classification

Medical imaging has seen significant advancements through machine learning, particularly convolutional neural networks (CNNs). These technologies have transformed the analysis of pathological images, enhancing the accuracy of diagnosing and classifying cellular anomalies. Digital pathology methodologies, including image analysis, have improved cervical cancer diagnostics. However, existing commercial platforms are often costly and restrictive, limiting customization and scalability.

CINNAMON-GUI is an open-source digital pathology tool based on CNNs for classifying Pap smear images. Transitioning to a Shiny app in Python, it offers enhanced user interface and interactivity. The application supports dynamic web interactions, advanced features for image analysis, and state-of-the-art CNN models tailored for digital pathology. Key features include intuitive UI components, real-time image and plot generation, memory-efficient data handling, and robust training capabilities with customizable CNN architectures. The tool also integrates with Labelme for defining regions of interest and allows testing on external biospecimens.

Model A (seed 42, 100 epochs) and Model B (same architecture with adjusted augmentation parameters) were compared. Model A stabilized with training accuracy around 0.88 and validation accuracy around 0.913. Model B showed improved performance with training accuracy around 0.91 and validation accuracy around 0.95. Feature mapping highlighted critical morphological aspects, improving classification accuracy. Model B reduced misclassification errors significantly compared to Model A.

CINNAMON-GUI demonstrates the potential of an open-source platform in digital pathology, providing transparency and collaborative opportunities. The tool enhances diagnostic accuracy through feature map analysis and optimized CNN training. Future development aims to extend its application to other cancer types, leveraging its dynamic and user-friendly interface for broader use in diagnostics.