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Updated: May 29, 2025
High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
Published on: July 6, 2012
Extraction of mannooligosaccharides from spent coffee grounds and its application for functional jelly with improved
Ae Eun Im1, Jung Choi2, Hyeon Seok Park2
1Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186 South Korea.
Abstract:
Mannooligosaccharides (MO) were extracted from spent coffee grounds (SCG) via combinational enzyme treatment with pectinase and mannanase from Aspergillus niger. Among 18 commercial enzymes to improve the accessibility of galactomannan, pectinase XL exhibited the highest yield, producing 235 mg hemicellulose/g of SCG. Pectinase treatment before mannanase resulted in coffee mannooligosaccharides (CMO) production 6.8 times higher than mannanase alone. This approach facilitated a 70% CMO extraction efficiency compared to the hemicellulose content present in the SCG. Additionally, HPLC analysis validated the composition of CMO, revealing 28.2% mannobiose (M2), 32.4% mannotriose (M3), 5.9% mannotetraose (M4), and 3.9% mannopentaose (M5). The CMO at different concentrations, along with fructose syrup, gelling agent, coffee extract, and coconut milk, was used to prepare jelly. CMO20 (20% CMO, w/w) jelly outperformed in both product stability and functional properties. The antioxidant activity of CMO20, measured by DPPH and FRAP assay, was 4.57 mM, with a Vit C equivalent and 15 mM FeSO4 equivalent, respectively. Furthermore, CMO20 showed 48% greater α-glucosidase inhibition than acarbose (5 mg/mL). CMO20 showed improved immunomodulatory effects with 4.7 and 16-fold higher nitric oxide and cyclooxygenase-2 content than methotrexate-suppressed RAW264.7 cells.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13197-024-06103-z.
