Related Experiment Video
Updated: May 29, 2025
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
Published on: October 5, 2015
Construction of a Subcutaneous Fat Transplantation Model Infected with Mycobacterium
Wuliang Diao1, Yang Hu1, Xiangyu Chen1
1Department of Plastic Surgery of Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Introduction:
Fat grafting procedures for body contouring and cosmetic reconstruction have received widespread attention.
Methods:
In recent years, there has been an increase in post-fat grafting infections caused by Mycobacterium abscessus (MA), and there is a lack of representative and standardized murine models of infection; therefore, there has been limited research on the treatment of post-fat grafting MA infections. To overcome this challenge, we constructed an MA infection model after fat grafting.
Results:
By evaluating skin charge, dermatopathology, and inflammatory markers, we found that the fat graft + 1×109 CFU/mL bacterial suspension infection group had significant inflammatory symptoms and elevated inflammatory factors on postoperative day 10.
Conclusion:
The model construction process was simple and reproducible, which paves the way for further studies on the impact of MA pathogenesis and the efficacy of new treatments.
