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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

298
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
298
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Chemically Specific Coherent Raman Imaging of Liquid-Liquid Phase Separation and Its Sequelae.

Alba M Arbiol Enguita1, Laurin Zöller2, Teemu Tomberg1

  • 1Division of Pharmaceutical Chemistry and Technology, University of Helsinki, Viikinkaari 5E, 00014 Helsinki, Finland.

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|February 7, 2025
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Summary

This study introduces a new imaging technique combining stimulated Raman scattering (SRS) and second harmonic generation (SHG) to visualize liquid-liquid phase separation (LLPS) and crystallization. This method offers real-time insights into drug crystallization processes.

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Pharmaceutical Science

Background:

  • Liquid-liquid phase separation (LLPS) is crucial for cellular organization and material crystallization.
  • LLPS impacts drug crystallization and delivery, but its characterization has lacked spatial and chemical resolution.
  • Understanding LLPS is vital across pharmaceutical, biomedical, food, and chemical industries.

Purpose of the Study:

  • To develop and apply a novel imaging technique for simultaneous characterization of LLPS and crystallization.
  • To investigate the interplay between LLPS and crystallization using ibuprofen as a model compound.
  • To explore the influence of dissolution medium and enantiomeric form on LLPS-driven crystallization.

Main Methods:

  • Utilized chemically specific stimulated Raman scattering (SRS) for molecular identification.
  • Employed second harmonic generation (SHG) for structural imaging.
  • Correlated SRS and SHG for real-time monitoring of phase separation and crystallization dynamics.

Main Results:

  • Successfully imaged crystallization within LLPS domains in real time.
  • Examined the influence of dissolution medium and enantiomeric form on LLPS and crystallization.
  • Discovered and partially characterized a new polymorph of (S)-ibuprofen.

Conclusions:

  • Correlative SRS and SHG provide unprecedented mechanistic insights into LLPS and crystallization.
  • This technique enables detailed analysis of spatial distribution, chemical composition, and crystal structure.
  • The findings have significant implications for pharmaceutical development and other chemical sectors.