Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson's disease
Shuyi Liu1, Wei Si2, Zhengbo Wang2
1State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
Abstract:
The lack of an etiologic Parkinson's disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis. We describe steps for localizing the substantia nigra and striatum using magnetic resonance imaging (MRI). We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum. For complete details on the use and execution of this protocol, please refer to Liu et al.1.
