Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.2K
RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Genomics02:02

Genomics

35.7K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.7K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.0K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.0K
Sanger Sequencing01:57

Sanger Sequencing

752.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
752.5K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A complete human pancreatic cancer genome.

bioRxiv : the preprint server for biology·2026
Same author

SpatialCOC: an integrative framework for spatial continuous mapping and cross-omics correction in spatial multi-omics data.

Nature communications·2026
Same author

Highly accurate ab initio gene annotation with ANNEVO.

Nature methods·2026
Same author

Population-level structural variant characterization using pangenome graphs.

Nature genetics·2026
Same author

A global view of human centromere variation and evolution.

bioRxiv : the preprint server for biology·2026
Same author

Genome diversity and signatures of natural selection in mainland Southeast Asia.

Nature·2025

Related Experiment Video

Updated: May 29, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K

Long and Accurate: How HiFi Sequencing is Transforming Genomics.

Bo Wang1,2, Peng Jia3, Shenghan Gao1,2

  • 1School of Automation Science and Engineering, Faculty of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Genomics, Proteomics & Bioinformatics
|February 7, 2025
PubMed
Summary

PacBio high-fidelity (HiFi) sequencing offers 99.9% accurate long reads, enabling detailed analysis of complex genomes and direct detection of base modifications. This technology advances genomics, transcriptomics, and epigenetics for precision medicine.

Keywords:
CentromereComplex genomic regionGenome assemblyLong-read sequencingVariant detection

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.3K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K

Related Experiment Videos

Last Updated: May 29, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.3K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K

Area of Science:

  • Genomics
  • Transcriptomics
  • Epigenetics

Background:

  • PacBio high-fidelity (HiFi) sequencing provides highly accurate, long single-molecule reads.
  • This circular consensus sequencing method achieves 99.9% accuracy, overcoming amplification biases.

Purpose of the Study:

  • To provide a comprehensive overview of HiFi sequencing applications and challenges.
  • To highlight the potential of HiFi sequencing in multi-omics research for advancing genetic understanding and precision medicine.

Main Methods:

  • Review of recent developments in PacBio HiFi sequencing technology.
  • Analysis of HiFi sequencing applications in genomics, transcriptomics, and epigenetics.

Main Results:

  • HiFi sequencing enables thorough analysis of complex genomic regions like tandem repeats and centromeres.
  • Direct detection of base modifications provides concurrent sequence and epigenetic data.
  • Streamlined genome assembly, variant detection, and full-length transcript analysis.

Conclusions:

  • HiFi sequencing significantly enhances genomic research capabilities.
  • This technology is pivotal for deepening the understanding of genetic mechanisms and advancing precision medicine through multi-omics integration.