Assessing perfluoroalkyl substance pollution in Central Mediterranean breeding shearwaters

Per- and polyfluoroalkyl substances (PFAS) are synthetic organofluorine compounds used in various products, which are highly durable in the environment and may pose risks to wildlife health. We investigated the blood cell concentrations of PFAS in breeding Scopoli's shearwaters (Calonectris diomedea) from three different colonies in the central and southern Mediterranean (Linosa, Malta, and La Maddalena). Shearwaters are flexible, high trophic level foragers, and foraging areas may differ according to sex and breeding stage. We examined inter- and intracolony differences in PFAS blood concentrations and compared them with exploited foraging areas and dietary tracers. Per- and polyfluoroalkyl substances were detected in all samples, with the major congeners detected in descending order being perfluoroctanesulfonic acid (PFOS), perfluoroundecanoic acid (PFuNA), perfluorododecanoic acid (PFDoDA), and perfluorotridecanoic acid (PFTriDA). The mean sum of PFAS during the chick-rearing phase was highest in the birds from Malta (145.1 ng/g dry wt, 95% confidence interval [CI] of the mean 106.8, 183.5) compared with Linosa (91.5 ng/g dry wt, 95% CI 72.9, 110.1) and La Maddalena (84.5 ng/g dry wt, 95% CI 61.7, 107.3), and the PFAS blood composition of shearwaters from La Maddalena and Malta differed. The PFAS concentrations in shearwaters from Linosa were higher during incubation than during chick-rearing, and males had higher PFAS concentrations than females during incubation. Some PFAS were associated with carbon and nitrogen stable isotope values. After baseline adjustment of stable isotope values, no differences were observed for adjusted δ15N and δ13C between the three colonies, suggesting that differences in PFAS levels attributed to diet were minor compared with regional differences. Our study highlights that shearwaters are useful biomonitors of PFAS exposure in remote marine areas.