Related Experiment Video
Updated: May 29, 2025
Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
Published on: May 27, 2022
Preparation, characterization, and induced human colon cancer HCT-116 and HT-29 cell apoptosis performance of
Ya-Hui Yu1, Kouadio Jean Eric-Parfait Kouame2, Xiaozhen Liu3
1School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China; Dongguan Key Laboratory of Typical Food Precision Design, China National Light Industry Key Laboratory of Healthy Food Development and Nutrition Regulation, School of Life and Health Technology, Dongguan University of Technology, Dongguan 523808, China.
Abstract:
The combination of selenium nanoparticles (SeNPs) with natural polysaccharides represents an effective strategy to address the limitations of SeNPs and providing a stable, highly bioactive form of Se. In this study, we explored the potential of longan polysaccharides (LP) in the preparation and stabilization of SeNPs. The morphology, stability, stabilization mechanism, and cytotoxicity of LP-SeNPs were investigated. The results showed that the LP-SeNPs were uniform spherical nanoparticles with a mean particle size of 100.4 ± 1.1 nm, and demonstrated excellent stability over a 14-day storage period, which was attributed to the interaction between the SeNPs and the hydroxyl groups present on the surface of LP. Meanwhile, LP-SeNPs have no toxic effect on rat intestinal epithelial (IEC-6) cells, while they are cytotoxic to human colon cancer (HCT-116 and HT-29) cells, including growth inhibition, morphological alteration, and the promotion of reactive oxygen species and mitochondrial membrane potential loss in a dose-dependent manner. LP-SeNPs induced apoptosis in HCT-116 and HT-29 cells by regulation of the apoptosis-related proteins including Bax, Bcl-2, caspase-3, and cytochrome c. Therefore, our results provide a theoretical basis for the development and application of LP and indicate the potential promise of LP-SeNPs as dietary Se supplements and health-promoting food ingredients.
