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Related Experiment Video

Updated: May 29, 2025

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
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Decoding maize meristems maintenance and differentiation: integrating single-cell and spatial omics.

Bin Li1, Wenhao Liu1, Jie Xu2

  • 1The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, Shandong Key Laboratory of Precision Molecular Crop Design and Breeding School of Life Sciences, Shandong University, Qingdao, Shandong 266237, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
|February 8, 2025
PubMed
Summary

Maize meristem research uses new single-cell and spatial technologies to understand how stem cells maintain and differentiate. This knowledge aids in developing higher-yielding maize varieties adapted to stress.

Keywords:
Maize meristemMeristem maintenanceRegulatory mechanismSingle-cell and spatial technologiesStem cell

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Area of Science:

  • Plant biology
  • Developmental genetics
  • Genomics

Background:

  • Plant organs develop from meristems, with the maize shoot apical meristem (SAM) forming above-ground structures.
  • Inflorescence meristems (IMs) in maize give rise to the tassel and ear.
  • Genetic studies have advanced understanding but face challenges due to maize's genome complexity.

Purpose of the Study:

  • To review recent advancements in maize meristem development.
  • To highlight the integration of single-cell and spatial technologies.
  • To explore mechanisms of stem cell maintenance and differentiation in SAM and IM.

Main Methods:

  • Review of genetic studies on maize meristem mutants.
  • Integration of single-cell and spatial technologies.
  • Analysis of gene regulatory networks and hormonal pathways.

Main Results:

  • Insights into stem cell maintenance and differentiation in maize SAM and IM.
  • Understanding of hormonal pathways and gene regulatory networks.
  • Cellular omics data reveal roles in stress response and adaptation.

Conclusions:

  • Single-cell and spatial technologies are crucial for maize meristem research.
  • Understanding meristem development can inform precision breeding.
  • Future research aims to enhance maize yield and stress adaptation.