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Related Concept Videos

Meiosis II02:02

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Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
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Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
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Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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UBE2D3 functions in mouse oocyte meiotic maturation.

Guorui Zhang1, Na Zhang1,2, Bin Zhang1

  • 1State Key Laboratory of Reproductive Medicine and Offspring Health, Changzhou Maternity and Child Health Care Hospital Changzhou Medical Center, Nanjing Medical University, Nanjing, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|February 8, 2025
PubMed
Summary

The E2 ubiquitin-conjugating enzyme UBE2D3 is crucial for mouse oocyte meiotic division. Its dysregulation contributes to age-related fertility decline, suggesting UBE2D3 as a therapeutic target.

Keywords:
UBE2D3aneuploidymaternal ageoocyte

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Area of Science:

  • Cell Biology
  • Reproductive Biology
  • Genetics

Background:

  • Ubiquitin-mediated proteolysis is vital for cell-cycle control during meiosis.
  • E2 ubiquitin-conjugating enzymes' roles in oocyte meiosis are not well understood.
  • Proper chromosome segregation relies on precise regulation of meiotic events.

Purpose of the Study:

  • To investigate the function of E2 ubiquitin-conjugating enzymes in mouse oocyte meiosis.
  • To identify key E2 enzymes involved in meiotic fidelity.
  • To explore the role of UBE2D3 in oocyte aging and fertility.

Main Methods:

  • Identified UBE2D3 as the most abundant E2 enzyme in mouse oocytes.
  • Utilized UBE2D3 depletion and overexpression in mouse oocytes.
  • Assessed meiotic progression, Cyclin B1 levels, kinetochore-microtubule attachments, spindle assembly checkpoint (SAC) function, and aneuploidy.
  • Examined oocytes from aged mice to correlate UBE2D3 levels with meiotic defects.

Main Results:

  • UBE2D3 is essential for meiotic division; depletion causes metaphase I arrest and Cyclin B1 accumulation.
  • UBE2D3 overexpression leads to reduced Cyclin B1, kinetochore-microtubule mis-attachments, SAC dysfunction, and aneuploidy.
  • Increased UBE2D3 in aged oocytes correlates with meiotic defects, partially reversible by UBE2D3 knockdown or Cyclin B1 overexpression.

Conclusions:

  • The UBE2D3-Cyclin B1 axis is critical for maintaining meiotic fidelity in oocytes.
  • UBE2D3 plays a significant role in age-related meiotic errors.
  • Targeting the UBE2D3-Cyclin B1 pathway may offer therapeutic strategies for improving oocyte quality and fertility in aging females.