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Updated: May 28, 2025
Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
Published on: May 10, 2021
Nonlinear ageing gero-marker dynamics of transcriptomic profile during calcific aortic valve mouse modeling
Hongzheng Li1, Xiaoshan Cui2, Zucheng Shang3
1National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China academy of Chinese Medical Sciences, Beijing, 100195, China; Postdoctoral Research Station, Guang'anmen Hospital, China Academy of Chinese Medical Science, Beijing, 100053, China.
Understanding ageing biomarker dynamics is key to managing degenerative heart disease. This study identified linear and nonlinear aging markers in a calcific aortic valve mouse model, highlighting accelerated aging periods.
Area of Science:
- Cardiovascular Biology
- Aging Research
- Genomics
Background:
- Degenerative heart disease management is challenging.
- Understanding aging biomarker dynamics can improve prevention and treatment.
- Calcific aortic valve disease (CAVD) is a common degenerative heart condition.
Purpose of the Study:
- To construct and analyze a calcific aortic valve mouse model at different ages.
- To investigate valve function degeneration and calcification patterns.
- To explore age-related gene expression changes and nonlinear dynamics using deep learning.
Main Methods:
- Development of a calcific aortic valve mouse model (C57BL/6 N).
- Assessment of valve function (peak transvalvular jet velocity) and calcification.
- RNA sequencing for gene expression analysis and deep learning for nonlinear dynamics investigation.
Main Results:
- Older mice exhibited impaired valve function and increased collagen/elastic fiber calcification.
- Gene expression analysis revealed 30 age-associated genes, with four showing significant upregulation.
- A substantial proportion of genes (52%) displayed nonlinear expression patterns with age.
Conclusions:
- The study identified both linear and nonlinear aging biomarkers in the calcific aortic valve mouse model.
- Specific age points, such as 48 weeks in mice, represent periods of accelerated aging.
- Findings provide insights into the complex dynamics of aging in degenerative heart disease.
