Localized assembly in biological activity: Origin of life and future of nanoarchitectonics

The concept of nanoarchitectonics has emerged as a post-nanotechnology paradigm in the field of functional materials development. This concept entails the construction of functional material systems at the nanoscale, based on the knowledge acquired from nanotechnology. In biological systems, advanced nanoarchitectonics is achieved through precise structural organization governed by spatial localization, a process facilitated by localized assembly mechanisms. A thorough understanding of the principles of localized assembly is crucial for the creation of complex, asymmetric, hierarchical organizations that are similar in structure and function to living organisms. This review explores the concept of localized assembly, highlighting its biological inspiration, providing representative examples, and discussing its contributions to nanoarchitectonics. Key examples include assemblies using biological materials, those mimicking cellular functions, and those occurring within cells. Additionally, the role of interfacial interactions and liquid-liquid phase separation in localized assembly is emphasized. Particularly, the utilization of liquid-liquid phase separation demonstrates a remarkable capacity for forming intricate compartmentalized structures without discernible membranes, paving the way for multifunctional, localized systems. These localized assemblies are fundamental to essential biological functions and provide valuable insights into the molecular mechanisms underlying the origin of cells and life. Such understanding holds significant promise for advancing materials nanoarchitectonics, particularly in biomedical applications.