Advances in fluorescent probes of non-alcoholic fatty liver disease

Non-alcoholic fatty liver disease (NAFLD) is the predominant chronic liver disease worldwide, with 20-30 % of individuals going on to develop non-alcoholic steatohepatitis (NASH), which could result in serious complications such as fibrosis, liver cirrhosis, and hepatocellular carcinoma. Since NAFLD is reversible in its early stages, early diagnosis is necessary. By using particular structural and functional designs, fluorescent probes can be made to detect NAFLD-related chemicals or biological processes with a high degree of sensitivity and selectivity. In this work, we summarize the existing fluorescent probes for identifying biomarkers in NAFLD, including microenvironment (viscosity, polarity), ROS, RNS, RSS, metal ions, enzymes, and RNA. Furthermore, future directions are envisioned to inform the creation of more accurate and reliable fluorescent probes for NAFLD diagnosis, emphasizing the benefits and challenges of fluorescence probes.