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Related Experiment Video

Updated: May 28, 2025

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

Hybrid next-generation sequencing protocol for testing HIV-2 drug resistance.

Fátima Gonçalves¹, Joaquim Cabanas¹, Inês Costa¹

¹Laboratório de Biologia Molecular (LMCBM, SPC, HEM, ULS-LO), Lisboa 1349-019, Portugal.

Journal of Virological Methods

|February 9, 2025

Summary

Next-generation sequencing (NGS) effectively detects drug resistance mutations in people living with HIV-2 (PLHIV-2), improving treatment monitoring. This advanced method identifies minority variants missed by Sanger sequencing, enhancing patient care.

Keywords:

DRM Hybrid NGS (Ion torrent)PLHIV-2 Sanger

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Last Updated: May 28, 2025

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

Published on: January 30, 2019

Area of Science:

Virology
Genetics
Clinical Diagnostics

Background:

HIV-2 infection impacts over a million individuals globally, with treatment complicated by inherent drug resistance.
Monitoring drug resistance mutations (DRM) is crucial for managing treatment failure in people living with HIV-2 (PLHIV-2).
Lack of commercial tests necessitates the development of in-house methods for DRM detection.

Purpose of the Study:

To evaluate a hybrid next-generation sequencing (NGS) Ion Torrent protocol for detecting DRM in PLHIV-2.
To assess the clinical utility of this NGS protocol in managing HIV-2 treatment.

Main Methods:

A hybrid NGS Ion Torrent protocol was used to analyze 100 samples from PLHIV-2 in Portugal.
Sanger sequencing was performed on 48 samples for comparative analysis.
NGS successfully amplified key viral regions: 92% protease, 91% reverse transcriptase, and 49% integrase.

Main Results:

NGS demonstrated high amplification rates for crucial HIV-2 drug resistance regions.
The NGS protocol showed strong statistical agreement with Sanger sequencing for DRM identification.
NGS identified two reverse transcriptase mutations (K70E, M184V) not detected by Sanger, highlighting its superior sensitivity for minority variants.

Conclusions:

Hybrid NGS is effective for detecting DRM in PLHIV-2, including minority variants.
This NGS protocol offers comprehensive data for adjusting antiretroviral therapy (ART) regimens.
Implementing NGS can improve patient outcomes and the quality of care for PLHIV-2.