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Annealing synchronizes the TOM complex with Tom7 in a new orientation

Liuyan Yang1, Mingdong Liu2, Lei Qi3

  • 1Marine Biotechnology Research Center, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China; School of Life Sciences, Department of Chemical Biology, Southern University of Science and Technology, Shenzhen, 518055, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China; Division of Healthy Oceans & Resource Sustainability, Laoshan Laboratory, Qingdao, 266237, China; Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, 518055, China.

Archives of Biochemistry and Biophysics
|February 9, 2025
PubMed
Summary

Annealing improves membrane protein structural determination. Fast cooling of the translocase of the outer mitochondrial membrane (TOM) complex to 0°C enhances resolution and reveals new conformations for protein translocation.

Keywords:
AnnealingMembrane proteinsThe TOM complex

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