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Updated: May 12, 2026

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
Published on: October 25, 2013
Comprehensive analysis of bacteriocins produced by clinical enterococcal isolates and their antibacterial activity
Ayumi Fujii1,2, Miki Kawada-Matsuo3,4, Mi Nguyen-Tra Le1
1Department of Oral and Maxillofacial Surgery, Hiroshima University Graduate School of Biomedical and Health Sciences, Kasumi1-2-3, Hiroshima City, Hiroshima, 734-8551, Japan.
Abstract:
It is well-known that Enterococcus species produce bacteriocins that have antibacterial activity. However, a comprehensive analysis of the bacteriocin distribution among Enterococcus strains has not been conducted. In this study, we identified the bacteriocin genes from 80 Enterococcus faecalis and 38 Enterococcus faecium strains and investigated their antibacterial activity. In the 80 E. faecalis strains, the cytolysin gene (61.3%), enterolysin A gene (27.5%) and BacL1 gene (45.0%) were identified. In the 38 E. faecium strains, the enterocin A gene (97.4%), enterocin B gene (2.6%), enterocin NKR-5-3B gene (21.0%), bacteriocin T8 gene (36.8%) and BacAS9 gene (23.7%) were identified. The antibacterial activity of all strains was tested against E. faecalis and E. faecium. The strains positive for the cytolysin, enterolysin A, BacL1, bacteriocin T8 or BacAS9 genes presented variable antibacterial activity. Several bacteriocin-positive strains showed antibacterial activity against other enterococcal species, but not against Staphylococcus or Escherichia coli. In addition, the enterolysin A-positive strain showed antibacterial activity against vancomycin-resistant E. faecium, and the bacteriocin T8- or BacAS9-positive strains showed activity against vancomycin-resistant E. faecalis and E. faecium. Our findings suggest that E. faecium and E. faecalis strains that carry different bacteriocin genes may affect the composition of the surrounding bacterial community.
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