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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 1, 2011
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A photo-elutable 8-17 DNAzyme labeling and PCR-free colorimetric quantification strategy for 5-hydroxymethylcytosine
Mingfeng Lai1, Shitao Lu1, Bao Huang1
1School of Medicine, Guangxi University of Science and Technology, Liuzhou, 545005, China. yuzhenning@gxust.edu.cn.
Analytical Methods : Advancing Methods and Applications
|February 10, 2025
Abstract:
We developed a PCR-free, cost-effective colorimetric assay using gold nanoparticles (AuNPs) to quantify 5-hydroxymethylcytosine (5hmC) without expensive MS instruments or antibodies. This method employs a photo-elutable 8-17 DNAzyme label specific to 5hmC sites, achieving accuracy comparable to ELISA when measuring 5hmC levels in various ICR mouse tissues.

