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LncRNA THUMPD3-AS1/microRNA-4465/KPNA2 axis impacts human hepatocellular carcinoma cell phenotypes
Jiawei Wang1, Chunzhong Qiao1, Baoyang Luo1
1Department of Hepatobiliary and Pancreatic Surgery, Taizhou People's Hospital Affiliated to Nanjing Medical University, Taizhou 225300, Mainland, China.
Regenerative Therapy
|February 10, 2025
Summary
Long non-coding RNA THUMPD3-AS1 promotes hepatocellular carcinoma (HCC) growth and migration. It does this by inhibiting microRNA-4465, which leads to increased KPNA2 expression and epithelial-mesenchymal transition (EMT).
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Hepatocellular carcinoma (HCC) is a significant global health concern.
- Long non-coding RNA THUMPD3-AS1 (lncRNA THUMPD3-AS1) is implicated in various cancers.
- The specific role of lncRNA THUMPD3-AS1 in HCC progression requires further elucidation.
Purpose of the Study:
- To investigate the role of lncRNA THUMPD3-AS1 in HCC.
- To explore the regulatory relationship between lncRNA THUMPD3-AS1, microRNA-4465 (miR-4465), and KPNA2 in HCC.
- To determine the impact of this pathway on HCC cell phenotype and epithelial-mesenchymal transition (EMT).
Main Methods:
- Analysis of clinical HCC specimens to quantify levels of lncRNA THUMPD3-AS1, KPNA2, miR-4465, and EMT markers.
- In vitro experiments involving HCC cell transfection with sh-THUMPD3-AS1 or miR-4465 mimic.
- Verification of molecular interactions and functional consequences on HCC cell behavior.
Main Results:
- lncRNA THUMPD3-AS1 and KPNA2 were overexpressed, while miR-4465 was reduced in HCC tissues.
- lncRNA THUMPD3-AS1 directly targets miR-4465, leading to KPNA2 upregulation.
- Silencing lncRNA THUMPD3-AS1 or restoring miR-4465 inhibited HCC cell proliferation, migration, and EMT.
Conclusions:
- lncRNA THUMPD3-AS1 promotes HCC cell growth and migration.
- This oncogenic effect is mediated by the lncRNA THUMPD3-AS1/miR-4465/KPNA2 axis.
- lncRNA THUMPD3-AS1 induces EMT in HCC cells by competitively inhibiting miR-4465 and upregulating KPNA2.
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