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Infrared Spectroscopy Coupled with Machine Learning Algorithms to Investigate Vascular Dysfunction in Ovariectomy: An

Tháfanys S Travezani1, Márcia H C Nascimento2, Tagana R da Cunha1

  • 1Department of Physiological Sciences, Federal University of Espirito Santo, Av. Mal. Campos, 1468 - Maruípe, Vitória 29047-105, Espírito Santo, Brazil.

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Summary
This summary is machine-generated.

Mid-infrared spectroscopy did not detect significant systemic changes in ovariectomized rats 15 days post-surgery. This study found no clear distinction between control and ovariectomized groups using FTIR analysis.

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Area of Science:

  • Biomedical Spectroscopy
  • Vascular Biology
  • Animal Models

Background:

  • Decreased female sex hormones impair vascular endothelium function, leading to disease.
  • Ovariectomy models are crucial for studying vascular dysfunction.
  • Fourier-transform infrared (FTIR) spectroscopy offers detailed molecular insights.

Purpose of the Study:

  • To evaluate systemic changes in an ovariectomy model using mid-infrared spectroscopy.
  • To determine if FTIR can differentiate between sham and ovariectomized rats based on serum profiles.
  • To assess vascular dysfunction markers post-ovariectomy.

Main Methods:

  • Analysis of 38 Wistar rat serum samples (18 SHAM, 20 OVX) 15 days post-ovariectomy.
  • Serum spectral data acquired using Bruker Alpha II FTIR equipment.
  • Unsupervised (PCA) and supervised (PLS-DA) analyses performed in MATLAB 2023.

Main Results:

  • Principal Component Analysis (PCA) did not reveal clear distinctions between OVX and SHAM groups.
  • Partial Least-Squares Discriminant Analysis (PLS-DA) showed 72.7% accuracy, but y-permutation tests indicated random results.
  • No significant systemic profile differences were detected between groups at 15 days post-surgery.

Conclusions:

  • Mid-infrared spectroscopy, using PCA and PLS-DA, could not identify significant systemic differences in rats 15 days after ovariectomy.
  • Further research may be needed to explore longer time points or different analytical approaches.
  • FTIR spectroscopy may not be sensitive enough to detect early systemic changes in this specific ovariectomy model.