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Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients
Salman M Toor1, Eman K Aldous1, Aijaz Parray2
1College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar.
Non-Coding RNA Research
|February 10, 2025
Summary
PIWI-interacting RNAs (piRNAs) are novel biomarkers for stroke. This study identified 10 differentially expressed piRNAs in acute ischemic stroke (AIS) and transient ischemic attack (TIA) patients, offering new diagnostic and prognostic potential.
Area of Science:
- Biochemistry
- Genetics
- Neurology
Background:
- Stroke is a leading cause of death and disability globally, often preceded by transient ischemic attack (TIA).
- Circulating non-coding RNAs (ncRNAs) show promise as stroke biomarkers.
- PIWI-interacting RNAs (piRNAs) have not been previously investigated in stroke diagnostics or prognostics.
Purpose of the Study:
- To explore circulating piRNAs as potential biomarkers for acute ischemic stroke (AIS) and TIA.
- To identify specific piRNAs differentially expressed in AIS and TIA patients.
Main Methods:
- Comprehensive circulating piRNA profiling using RNA-sequencing on serum samples from AIS and TIA patients.
- Stratification of the cohort into discovery and cross-validation datasets for robust piRNA identification.
- Comparison of piRNA expression levels between AIS, TIA, and healthy control groups.
Main Results:
- A panel of 10 differentially regulated piRNAs was identified between AIS and TIA patients.
- Eight piRNAs (hsa-piR-28272, -piR-32972, -piR-28247, -piR-24553, -piR-24552, -piR-28275, -piR-28707, -piR-32882) were upregulated in AIS.
- Two piRNAs (hsa-piR-23058, -piR-23136) were downregulated in AIS, with all 10 showing differential expression compared to healthy controls.
Conclusions:
- Circulating piRnome alterations are associated with ischemic brain injury in AIS and TIA patients.
- These findings suggest piRNAs as potential diagnostic and prognostic biomarkers for stroke.
- Further research into piRNAs may offer new strategies to reduce stroke-related mortality and morbidity.
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