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Omeprazole with Calcium Hydroxide's Effectiveness as an Intracanal Medication against Enterococcus faecalis
Binila Surendra Babu1, Souparnika Divakaran Padmini2, Manthra Ajith Shreya1
1Department of Conservative Dentistry and Endodontics, Al Azhar Dental College, Thodupuzha, Kerala, India.
Aims:
To evaluate the effectiveness of omeprazole mixed with calcium hydroxide (Ca(OH)2), as intracanal medicament in eliminating Enterococcus faecalis.
Materials And Methods:
Our study included 93 extracted mandibular premolars. A total of 15 μL of 0.5 McFarland standard E. faecalis (ATCC 29212) was inoculated into each canal and incubated at 36.5°C for 24 h. The specimens were divided into three groups: Group I (n = 30), control group without intracanal medicament, Group II (n = 30) with Ca(OH)2 and Group III (n = 30) with omeprazole and Ca(OH)2. The numbers of colony forming units (CFUs) were counted after 24 h. The same procedure was repeated after 7 and 14 days for the remaining samples.
Statistical Analysis Use:
Statistical analysis was performed using the analysis of variance (ANOVA) test (P < 0.05).
Results:
We found a significant reduction in the colony-forming unit count in the omeprazole- Ca(OH)2 group. In the Ca(OH)2 group, the number of CFUs was significantly higher than in the omeprazole-Ca(OH)2 group.
Conclusion:
Omeprazole mixed with Ca(OH)2 was more effective than Ca(OH)2 alone in eliminating E. faecalis.
Insights
Omeprazole combined with calcium hydroxide (Ca(OH)2) effectively eliminates Enterococcus faecalis. This combination therapy shows superior antimicrobial activity compared to calcium hydroxide alone in root canals.
Area of Science:
- Endodontics
- Microbiology
- Materials Science
Background:
- Enterococcus faecalis is a common pathogen in persistent root canal infections.
- Intracanal medicaments are crucial for microbial control during endodontic treatment.
- Calcium hydroxide (Ca(OH)2) is a widely used intracanal medicament, but its efficacy against E. faecalis can be limited.
Purpose of the Study:
- To evaluate the efficacy of omeprazole combined with calcium hydroxide (Ca(OH)2) as an intracanal medicament.
- To compare the antimicrobial activity of omeprazole-Ca(OH)2 mixture against E. faecalis with Ca(OH)2 alone.
Main Methods:
- Ninety-three extracted mandibular premolars were inoculated with E. faecalis.
- Specimens were divided into three groups: control, Ca(OH)2 alone, and omeprazole-Ca(OH)2 mixture.
- Colony-forming units (CFUs) were counted at 24 hours, 7 days, and 14 days post-treatment.
Main Results:
- A significant reduction in E. faecalis CFUs was observed in the omeprazole-Ca(OH)2 group.
- The omeprazole-Ca(OH)2 group demonstrated significantly lower CFU counts compared to the Ca(OH)2 alone group.
- Statistical analysis using ANOVA (P < 0.05) confirmed the significant differences.
Conclusions:
- Omeprazole mixed with Ca(OH)2 is more effective in eliminating E. faecalis than Ca(OH)2 alone.
- This combination therapy presents a promising approach for managing E. faecalis in endodontic treatments.
- Further research may explore the synergistic mechanisms and clinical applications of this combined medicament.
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