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Antimicrobial Effectiveness of Endodontic Irrigants Against E. Faecalis: An In vitro Study
Ajay Chhabra1, B Saravana Prathap2, K P Ramya2
1Department of Dentistry, AIIMS Kalyani, West Bengal, India.
Aim:
This in vitro study compared the antimicrobial efficacy of 5.25% sodium hypochlorite, 10% citric acid, and 3% hydrogen peroxide against Enterococcus faecalis (E. faecalis), using normal saline as a control.
Method:
E. faecalis isolates were exposed to different irrigants, with bacterial colony counts measured at various intervals using the spread plate method.
Results:
Sodium hypochlorite and citric acid effectively inhibited E. faecalis, while hydrogen peroxide was less effective. Sodium hypochlorite dissolved tissues and acted germicidally; citric acid disrupted bacterial structures through low pH. Hydrogen peroxide's effect was due to free radical formation and oxygen release.
Conclusion:
Both 5.25% sodium hypochlorite and 10% citric acid showed equal efficacy against E. faecalis, while hydrogen peroxide was less effective. Further research should explore clinical applications and polymicrobial biofilms.
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