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Bone Remodeling and Repair01:31

Bone Remodeling and Repair

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

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Related Experiment Video

Updated: Jul 26, 2026

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
10:19

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Domestic Chicken Eggshell-Derived Bone Substitute: Synthesis, Characterization and In Vitro Cell Viability.

Sruthy Prathap1, K S Rajesh2, Nebu G Thomas3

  • 1Department of Periodontology, Yenepoya Dental College, Mangalore, Karnataka, India.

Journal of Pharmacy & Bioallied Sciences
|February 10, 2025
PubMed
Summary

Chicken eggshells were transformed into hydroxyapatite (HA), a bone-like biomaterial. This synthesized HA is non-toxic and supports cell growth, indicating potential for biomedical uses.

Keywords:
Cell viabilitycharacterizationeggshellhydroxyapatite

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Area of Science:

  • Biomaterials Science
  • Biomineralization
  • Materials Chemistry

Background:

  • Hydroxyapatite (HA) exhibits high bioactivity and chemical resemblance to bone tissue.
  • HA can be sourced from natural or synthetic origins for various applications.

Purpose of the Study:

  • To synthesize hydroxyapatite (HA) from domestic chicken eggshells.
  • To characterize the synthesized HA using advanced analytical techniques.
  • To evaluate the in vitro cell viability of the synthesized HA.

Main Methods:

  • Chemical precipitation method used for HA synthesis from chicken eggshells at 900°C.
  • Characterization via FTIR, XRD, SEM, and EDS.
  • In vitro cell viability assessed using MTT assay.

Main Results:

  • FTIR and EDS confirmed the presence of calcium and phosphate ions.
  • XRD analysis demonstrated the crystalline nature of the synthesized HA.
  • SEM revealed particles <100 μm with a porous structure; MTT assay showed non-cytotoxicity and favorable cell growth.

Conclusions:

  • Synthesized HA from chicken eggshells is crystalline and exhibits good cell viability.
  • The material is non-cytotoxic, supporting its potential for biomedical applications.
  • Eggshell-derived HA offers a promising biomaterial for bone regeneration and tissue engineering.