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Updated: May 28, 2025

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

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Substrate Trapping in Polyketide Synthase Thioesterase Domains: Structural Basis for Macrolactone Formation.

Tyler M McCullough1,2, Vishakha Choudhary1,2, David L Akey1

  • 1Life Sciences Institute, Mary Sue Coleman Hall, 210 Washtenaw Ave., University of Michigan, Ann Arbor, MI 48109-2216, United States.

ACS Catalysis
|February 10, 2025
PubMed
Summary

Researchers engineered thioesterases (TEs) using 1,3-diaminopropionic acid (DAP) to trap intermediates, revealing how these enzymes form macrolactone antibiotics. This work aids in developing new biocatalysts for drug discovery.

Keywords:
Macrolactonizing enzymePolyketide synthaseSubstrate trappingThioesteraseUnnatural amino acid incorporationX-ray crystallography

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