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Updated: May 28, 2025
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Anti-MRSA effects of synergism of recombinant lysostaphin with biosynthesized silver nanoparticles
Zeena Fouad Saleh1, Balsam Miri Mizher2
1Unit of Zoonotic Disease Research, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq.
Background:
Methicillin-resistant Staphylococcus aureus (MRSA) strains become a major challenge to public health and health provider due to their wide spread and resistance to wide spectrum of antibacterial agents.
Aim:
This study aimed to characterize recombinant lysostaphin produced by Escherichia coli BL21(DE3) and to investigate the biosynthesis of silver nanoparticles (SNPs) by Candida albicans against MRSA.
Methods:
Samples were collected from vaginal samples. The methods included efficient cloning, expression, and purification of recombinant lysostaphin, a potent antibacterial enzyme targeting S. aureus, and achieved with advanced molecular techniques in E. coli BL21(DE3). The SNPs were synthesized using a biogenic approach with C. albicans, demonstrating stable and efficient silver ion reduction, as confirmed by ultraviolet-visible spectroscopy, scanning electron microscope, and Fourier-transform infrared spectroscopy (FTIR) analyses.
Results:
The antibacterial assays against MRSA strains of S. aureus showed significant inhibition by both lysostaphin and SNPs. The combination of biosynthesized SNPs/polyethylene glycol with lysostaphin exerted significant activity against tested bacteria, resulting in zone of growth inhibition of 20.33 ± 0.88 mm.
Conclusion:
These findings suggest a promising synergistic antibacterial strategy, offering hope in the fight against MRSA. The study findings reveal that lysostaphin and SNPs could be effective in the treatment of MRSA-related infections, especially when used in combination.
Insights
This study explored recombinant lysostaphin and biosynthesized silver nanoparticles (SNPs) for combating Methicillin-resistant Staphylococcus aureus (MRSA). Their combination showed significant antibacterial activity, offering a promising strategy against MRSA infections.
Area of Science:
- Microbiology
- Nanotechnology
- Biochemistry
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat due to widespread prevalence and antibiotic resistance.
- Existing antibacterial agents are often ineffective against MRSA strains.
Purpose of the Study:
- To characterize recombinant lysostaphin produced in Escherichia coli.
- To investigate the biosynthesis of silver nanoparticles (SNPs) using Candida albicans.
- To evaluate the efficacy of lysostaphin and SNPs, individually and in combination, against MRSA.
Main Methods:
- Recombinant lysostaphin was cloned, expressed, and purified using molecular techniques in E. coli BL21(DE3).
- Silver nanoparticles (SNPs) were biosynthesized by C. albicans via biogenic reduction of silver ions.
- Characterization of SNPs involved UV-Vis spectroscopy, SEM, and FTIR analysis.
Main Results:
- Both recombinant lysostaphin and biosynthesized SNPs demonstrated significant antibacterial activity against MRSA strains.
- The combination of SNPs/polyethylene glycol with lysostaphin resulted in a notable zone of growth inhibition (20.33 ± 0.88 mm).
Conclusions:
- Lysostaphin and SNPs show potential as effective agents for treating MRSA infections.
- A synergistic antibacterial strategy using lysostaphin and SNPs offers a promising approach to combat MRSA.
- This combination therapy could be a valuable tool in addressing the challenge of antibiotic-resistant bacteria.
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