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Updated: May 28, 2025

A Macrophage-Tumor Spheroid Co-Invasion Assay

A Macrophage-Tumor Spheroid Co-Invasion Assay

Published on: January 24, 2025

A Macrophage-Tumor Spheroid Co-Invasion Assay.

Sven Hey¹, Stefan Linder²

¹Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf.

Journal of Visualized Experiments : Jove

|February 10, 2025

Summary

This study introduces a new in vitro method to assess how human macrophages affect non-small cell lung cancer cell invasion. The novel co-cultivation technique uses tumor spheroids in a 3D matrix to analyze cancer cell metastasis.

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Area of Science:

Oncology
Immunology
Cell Biology

Background:

Cancer metastasis is significantly influenced by immune cell interactions.
Existing in vitro models have limitations in replicating in vivo cancer cell invasion dynamics.
Understanding macrophage-cancer cell interactions is crucial for developing anti-metastatic therapies.

Purpose of the Study:

To develop and present a novel in vitro co-cultivation system for studying cancer cell invasion.
To investigate the impact of human macrophages on the 3D invasion of non-small cell lung carcinoma cells.
To evaluate the effect of macrophage regulatory factor depletion on cancer cell invasiveness.

Main Methods:

Utilized H1299 non-small cell lung carcinoma tumor spheroids embedded in a 3D collagen I matrix.

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Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Published on: June 23, 2020

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

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Last Updated: May 28, 2025

A Macrophage-Tumor Spheroid Co-Invasion Assay

A Macrophage-Tumor Spheroid Co-Invasion Assay

Published on: January 24, 2025

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Published on: June 23, 2020

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

Co-cultivated tumor spheroids with human macrophages in a novel in vitro setup.

Employed small interfering RNA (siRNA) to deplete regulatory factors in macrophages and assessed cancer cell invasion parameters (spheroid area, number of invading cells).

Main Results:

The developed method allows for the quantitative assessment of cancer cell invasion from tumor spheroids.
Differences in cancer cell invasion were detected when macrophage regulatory factors were depleted.
The system provides measurable parameters to analyze the complex interactions influencing metastasis.

Conclusions:

The novel 3D co-cultivation model effectively approximates in vivo conditions for studying cancer cell invasion.
This method enables the investigation of immune cell modulation's impact on cancer metastasis.
The approach offers insights into potential therapeutic targets for reducing cancer cell invasiveness.