Related Experiment Video

Updated: May 28, 2025

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum
06:12

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

1.6K

Outer membrane vesicles from Pseudomonas aeruginosa induce autophagy-regulated pyroptosis in THP-1 cells

Jing Ge1,2, Yaoyang Liu1,2, Tianqi Wu3

  • 1Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, P.R. China.

Archives of Microbiology
|February 10, 2025
PubMed

Insights

Pseudomonas aeruginosa outer membrane vesicles (PA-OMVs) impact immune responses by modulating autophagy and pyroptosis. Inhibiting autophagy reduced pyroptosis, suggesting a therapeutic target for infections.

Area of Science:

  • Microbiology and Immunology
  • Cellular Biology
  • Pathogen-Host Interactions

Background:

  • Outer membrane vesicles (OMVs) from Pseudomonas aeruginosa (PA-OMVs) are key virulence factors involved in immune modulation and inflammation.
  • Autophagy and pyroptosis are critical cellular processes regulating immune responses and host defense against infections.
  • The interplay between PA-OMVs, autophagy, and pyroptosis is not well understood, hindering the development of targeted therapies.

Purpose of the Study:

  • To investigate the influence of PA-OMVs on cellular autophagy and pyroptosis.
  • To elucidate the regulatory mechanisms governing these interactions.
  • To identify potential therapeutic strategies for infectious diseases by targeting the autophagy-pyroptosis axis.

Main Methods:

  • Bulk RNA sequencing and bioinformatics analysis of PA-OMV-treated cells.
  • Utilizing autophagy inhibitors (chloroquine, 3-methyladenine) to assess pyroptosis.
  • Employing RT-PCR and ELISA to quantify pyroptosis levels.

Main Results:

  • PA-OMVs were found to modulate key immune and inflammatory pathways.
  • A complex interplay between autophagy and pyroptosis was observed.
  • Inhibition of autophagy led to decreased expression of pyroptosis markers, indicating a regulatory role.

Conclusions:

  • The autophagy-pyroptosis axis is significantly influenced by PA-OMVs.
  • Targeting this axis presents a promising avenue for novel infection control strategies.
  • Findings support the potential for developing new vaccines and therapeutics based on modulating these cellular processes.
Keywords:
Pseudomonas aeruginosaAutophagyOuter membrane vesiclesPyroptosisRNA sequencing

More Related Videos

Monitoring Stub1-Mediated Pexophagy08:26

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

1.5K
In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

17.5K

Related Concept Videos

Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.1K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.1K
Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.1K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
3.5K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.5K