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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: May 28, 2025

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

Curcumin Protects Human Sperm Quality During Cryopreservation.

Banafsheh Mansouri-Bahrani¹, Maryam Azizi Kutenaee², Mitra Heydari Nasrabadi¹

¹Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.

Biopreservation and Biobanking

|February 10, 2025

Summary

Curcumin pre-treatment significantly improves frozen-thawed human sperm quality by enhancing motility and vitality. The optimal concentration of 50 μM curcumin reduces reactive oxygen species (ROS) and preserves sperm morphology, mitigating cryopreservation damage.

Keywords:

DNA fragmentation ROS cryopreservation curcumin sperm

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Last Updated: May 28, 2025

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Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation

Published on: January 28, 2018

Area of Science:

Reproductive Biology
Biochemistry

Background:

Cryopreservation of human sperm can lead to reduced fertility due to oxidative stress and physical damage.
Antioxidants are increasingly studied for their potential to mitigate cryopreservation-induced sperm injury.

Purpose of the Study:

To investigate the protective effects of curcumin pre-treatment on human sperm parameters following cryopreservation.
To determine the optimal concentration of curcumin for preserving sperm quality after thawing.

Main Methods:

Human semen samples from normozoospermic men were divided into fresh and cryopreserved groups.
Sperm were pre-treated with varying concentrations of curcumin (0, 20, 50, 100 μM) before freezing.
Post-thaw analysis included sperm motility, morphology, vitality, DNA fragmentation, and reactive oxygen species (ROS) levels.

Main Results:

Cryopreservation significantly impaired sperm quality.
50 μM curcumin pre-treatment significantly improved progressive motility, vitality, and sperm morphology.
This concentration also led to a significant reduction in ROS levels and a trend towards decreased DNA fragmentation.

Conclusions:

Curcumin effectively ameliorates cryopreservation-induced damage to human sperm.
A concentration of 50 μM curcumin shows the most promising results for preserving sperm quality post-thaw.
Curcumin represents a potential therapeutic agent for improving sperm cryosurvival rates.