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Ion Mobility-Coupled Mass Spectrometry for Metallophore Detection.

Bailey A Bell1, Josephine M Anderson1, Scott R Rajski1

  • 1Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.

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We developed a novel method using ion mobility-coupled mass spectrometry (IM-MS) to rapidly detect metal-binding molecules (metallophores) in complex bacterial extracts. This technique improves sensitivity and metal selectivity for identifying natural products.

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Area of Science:

  • Analytical Chemistry
  • Natural Products Chemistry
  • Microbiology

Background:

  • Metallophores are crucial for microbial interactions and bacterial survival.
  • Existing metallophore identification methods suffer from low sensitivity, poor metal selectivity, and complex data analysis.

Purpose of the Study:

  • To develop a novel, sensitive, and selective method for detecting metallophores in natural product extracts.
  • To overcome limitations of current metallophore identification techniques.

Main Methods:

  • Utilized ion mobility-coupled mass spectrometry (IM-MS) for metallophore detection.
  • Compared direct infusion (DI) and liquid chromatography (LC) separation coupled with IM-MS.
  • Analyzed marine bacterial extracts with and without added metals to identify metal-binding metabolites by changes in mass and mobility.

Main Results:

  • Developed a novel IM-MS approach for metallophore detection based on mass and mobility shifts.
  • DI-IM-MS generally outperformed LC-IM-MS in speed and simplicity, though LC improved detection in some cases by reducing ion suppression.
  • Identified 10 metallophores in a marine *Micromonospora* sp. extract.

Conclusions:

  • IM-MS provides a rapid and effective method for detecting metal-binding natural products in complex biological samples.
  • The comparison of mass and mobility data with and without metals enables specific metallophore identification.