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PAM-adjacent DNA flexibility tunes CRISPR-Cas12a off-target binding

Aleique Allen1, Brendon H Cooper2,3, Jaideep Singh1

  • 1Department of Chemistry, University of Southern California, 3430 S Vermont Ave., Los Angeles, CA, 90089, USA.

Scientific Reports
|February 10, 2025
PubMed
Summary

DNA flexibility near the protospacer-adjacent motif (PAM) influences Cas12a nuclease off-target binding. Unpairing at PAM+1, +2, and +3 positions increases Cas12a off-target activity, impacting genome editing applications.

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