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circAFF2 promotes the development of AML by binding to PML mRNA
Lei Yao1, Xinyang Zhang2, Xiaoqing Li3
1College of Biomedicine and Health, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Oncogene
|February 10, 2025
Summary
Circular RNA circAFF2 is elevated in acute myeloid leukemia (AML). Inhibiting circAFF2 reduces AML cell growth and promotes cell death, offering potential diagnostic and therapeutic targets for AML.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Acute myeloid leukemia (AML) pathogenesis involves complex molecular mechanisms.
- The role of circular RNAs (circRNAs) in AML remains incompletely understood.
- Identifying novel molecular regulators is crucial for AML research.
Purpose of the Study:
- To systematically identify differentially expressed circRNAs in AML.
- To investigate the functional role of circAFF2 in AML progression.
- To elucidate the molecular mechanism by which circAFF2 regulates AML.
Main Methods:
- High-throughput sequencing of clinical AML and healthy donor samples.
- In vitro functional assays (cell proliferation, apoptosis) following circRNA manipulation.
- In vivo studies using mouse models of AML.
- Molecular mechanism studies involving mRNA binding and degradation analysis.
Main Results:
- circAFF2 was significantly upregulated in AML patient samples compared to healthy controls.
- Knockdown of circAFF2 inhibited AML cell proliferation and induced apoptosis.
- Overexpression of circAFF2 promoted AML cell proliferation and survival.
- In vivo experiments demonstrated that circAFF2 knockdown slowed tumor growth and prolonged survival in mice.
- circAFF2 was found to bind PML mRNA, promoting its degradation and affecting AML cell function.
Conclusions:
- circAFF2 is a key regulator in AML development and progression.
- circAFF2 promotes AML by targeting PML mRNA degradation.
- circAFF2 represents a potential biomarker and therapeutic target for acute myeloid leukemia.
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